Multiplex PCR for simultaneous detection of 3 major fish pathogens incriminated in bacterial septicemic syndrome
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چکیده
منابع مشابه
Multiplex PCR assay for detection of bacterial pathogens associated with warm-water Streptococcosis in fish.
A multiplex PCR-based method was designed for the simultaneous detection of the main pathogens involved in warm-water streptococcosis in fish (Streptococcus iniae, Streptococcus difficilis, Streptococcus parauberis, and Lactococcus garvieae). Each of the four pairs of oligonucleotide primers exclusively amplified the targeted gene of the specific microorganism. The sensitivity of the multiplex ...
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Infections with Pasteurella multocida, Salmonella enterica, Riemerella anatipestifer, and Escherichia coli result in high morbidity and mortality, which cause significant economic loss in the poultry industry. It can be difficult to distinguish these pathogens based on clinical signs because these pathogens can cause similar clinical signs and coinfections can occur. Thus, rapid and sensitive d...
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Accepted: 11/07/2014 Abstract The study was carried out to standardize a multiplex PCR assay for the simultaneous detection of four common food pathogens such as Listeria monocytogenes, Yersinia enterocolitica, Staphylococcus aureus and Salmonella enterica Typhimurium. Pathogen-specific DNA primers targeting hly A, ail, nuc and stm genes were employed for the identification of L. monocytogenes,...
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ژورنال
عنوان ژورنال: Journal of Veterinary Medical Research
سال: 2018
ISSN: 2357-0520
DOI: 10.21608/jvmr.2018.43311